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cells were isolated from day P3 postnatal rat brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS Dissociator, CD11b/c (Microglia) MicroBeads, a MiniMACS™ Separator, and two MS Columns. Cells were fluorescently stained with CD11b/c-FITC (# 130-105-273) in combination with CD45-APC and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before MicroBead labeling
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