Lineage Cell Depletion Kit, human

Lineage Cell Depletion Kit, human

The Lineage Cell Depletion Kit is an indirect magnetic labeling system for the depletion of mature hematopoietic cells such as T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, erythroid cells, and their committed precursors from bone marrow aspirate, cord blood, or mobilized leukapheresis product. The depletion of lineage-committed cells results in the enrichment of untouched stem and progenitor cells.

Detailed separation procedure

Cells are first labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens (CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a (Glycophorin A)). These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells.

Downstream applications

  • Flow cytometric characterization of rare stem and progenitor cell subsets
  • Evaluation of hematopoietic differentiation potential in vitro using CFU assays (CFC, CFU-GM, BFU-E, and CFU-Mk)
  • Evaluation of the CFU-F potential

Columns

MS, LS, XS, or autoMACS
®
Columns.
  • Selected references

    1. Mizokami, T. et al. (2009) Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells. Haematologica 94: 618-628
    2. Schreiber, T. D. et al. (2009) The integrin alpha9beta1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation. Haematologica 94: 1493-1501
Product options: 1
for 1×
10
9
total cells
-

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