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Mouse ES cell cultures containing 14% early differentiated cells (A), as they can occur during feeder-free cultivation, were separated using the Pluripotent Stem Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator. Differentiated cells were completely removed resulting in a purity of approx. 99% pluripotent cells (B). Flow cytometric analysis using the MACSQuant® Analyzer. Debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.
Phase contrast pictures of fixed feeder-free mouse embryonic stem cell cultures one day after plating without (A) and with separation (B) indicate efficient removal of differentiated cells.
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