Anti-SSEA-1 (CD15) MicroBeads, human and mouse

Anti-SSEA-1 (CD15) MicroBeads, human and mouse

Anti SSEA-1 (CD15) MicroBeads are the first product to allow magnetic cell separation based on the expression of SSEA-1. In less than an hour, it is possible to
  • separate pluripotent mouse ES or iPS cells from heterogeneous cultures, e.g. to eliminate feeder cells,
  • enrich reprogrammed mouse iPS cells,
  • deplete unwanted pluripotent cells from ES or iPS cell-derived differentiation cultures,
  • deplete human ES or iPS cells that have undergone spontaneous differentiation.

Background information

In the murine system, SSEA-1 is one of the key embryonic stem (ES) cell markers associated with the state of pluripotency. It is known to be expressed on undifferentiated murine ES and induced pluripotent stem (iPS) cells.
1-4
In contrast, SSEA-1 is not expressed on undifferentiated human ES cells but becomes upregulated during spontaneous differentiation.
5

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Durcova et al. (1998) J. Reprod. Dev. 44: 85-89
    2. Cui, L. et al. (2004) Spatial distribution and initial changes of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells before and during differentiation. J. Histochem. Cytochem. 52: 1447-1457
    3. Shin, S. et al. (2007) Stage-dependent Olig2 expression in motor neurons and oligodendrocytes differentiated from embryonic stem cells. Stem Cells Dev. 16: 131-141
    4. Stadtfeld et al. (2008) Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell 6: 230-240
    5. International Stem Cell Initative (2007) Nat. Biotechnol. 25(7): 151-159
Product options: 1
for 1×
10
9
total cells
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