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Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2Kk
were taken either before or after MACS®
Separation and stained with anti-H-2Kk
-PE before analysis by flow cytometry. Expression of ectopic H-2Kk
was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation.
Isolated mouse ES cells
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