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Endothelial cells were isolated from adult CD-1®
rat lung by using the Lung Endothelial Cell Isolation Kit, one LD Column with MidiMACS™ Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with Labeling Check Reagent-VioBlue®
and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Enriched lung endothelial cells
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