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Separation of CD209+
cells from human PBMCs spiked with 1% Mo-DCs using the CD209 MicroBead Kit, two MS Columns, and a MiniMACS™ Separator. Mo-DCs were generated in vitro with GM-CSF and IL-4 for 7 days. Cells were stained with CD209 (DC-SIGN)-FITC and CD304 (BDCA-4/Neuropilin-1)-PE and analyzed by flow cytometry.
Cells depleted of
Isolated CD209 (DC-SIGN)+
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