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PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained according to secretion of IL-22 using the IL-22 Secretion Assay - Cell Enrichment and Detection Kit (PE), human. Displayed is the analysis of secreted IL-22 (PE) versus CD154-APC of viable CD4+
T cells (gated on CD4-FITC). B cells, monocytes, and dead cells were excluded according to CD20-PerCP, CD14-PerCP, and PI-staining respectively.
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