Mouse IL-6

Mouse IL-6

IL-6 stands for interleukin 6. Mouse IL-6 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-6 can be used for a variety of applications, including:
  • Induction of colony formation from hematopoietic progenitor cells in semi-solid medium.
  • Replacement of feeder cells in the preparation of murine hybridomas.
  • In vitro differentiation of TH 17 cells.
  • Cultivation of bone marrow cells for retroviral transduction.

Background information

Interleukin 6 (IL-6), originally identified as a B cell differentiation factor, is a multifunctional cytokine which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces, for instance, the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, macrophages, fibroblasts, endothelial cells, and T cells. Disturbed IL-6 production has been associated with pathological processes, including inflammatory autoimmune diseases and cancer.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 7TD1 cells (NIBSC 93/730)
  • premium grade: ≥ 4×
    10
    8
    U/mg
  • research grade: ≥ 2×
    10
    8
    U/mg
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
KRW 249,800.00
E. coli
25 µg
KRW 474,500.00
E. coli
10 µg
KRW 308,000.00
E. coli
25 µg
KRW 599,400.00
E. coli
100 µg
KRW 1,032,300.00
E. coli
1000 µg
KRW 4,487,200.00