Human IL-7

Human IL-7

IL-7 stands for interleukin 7. Human IL-7 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-7 can be used for a variety of applications, including:
  • In vitro T cell expansion.
  • In vitro T cell priming.
  • In vitro differentiation of T cells and iNKT cells.
  • Investigation of IL-7 mediated signaling pathways.

Background information

IL-7 is a member of the type I cytokine family. The primary sources of IL-7 are non-hematopoietic stromal cells in bone marrow, thymus, and lymphoid organs and tissues. It is a pleiotropic cytokine with central roles in modulating T cell development and peripheral T cell homeostasis. IL-7 can act both as a T cell growth factor as well as a critical anti-apoptotic survival factor for naive and memory T cells. IL-7 is related to IL-2 and signals through a heterodimeric receptor composed of the common cytokine signaling γ-chain and IL-7 receptor α-chain.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 2E8 cells (NIBSC 90/530)
  • premium grade: ≥ 5×
    10
    7
    U/mg
    (typical activity: ≥ 1.5×
    10
    8
    U/mg
    )
  • research grade: ≥ 2×
    10
    7
    U/mg
  • Selected references

    1. Engelmann S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
    2. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
    3. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
    4. Dussiau C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
    5. Ishihara, K. et al. (1991) Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B-to B-cell transition. Dev. Immunol. 1: 149-161
    6. Dietz, L. et al. (2010) Tracking human contact allergens: from mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T-cell priming. Toxicol. Sci. 117(2): 336-347
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
KRW 249,800.00
E. coli
25 µg
KRW 474,500.00
E. coli
10 µg
KRW 308,000.00
E. coli
25 µg
KRW 599,400.00
E. coli
100 µg
KRW 1,373,600.00
E. coli
1000 µg
KRW 5,353,000.00

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