Human IL-27

Human IL-27

IL-27 stands for interleukin 27. Human IL-27 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human Interleukin 27 can be used for a variety of applications, including:
  • Research on autoimmune diseases.
  • Cancer Research.
  • Study of dendritic cell development.

Background information

Interleukin 27 (IL-27) is a heterodimeric protein consisting of EBI-3 (IL-27B) and IL-27p28 (IL-27A). IL-27 forms together with IL-12, IL-23, and IL-35 a family of heterodimeric proteins. Produced as an early product of antigen-presenting cells (APC), IL-27 plays a role in the adaptive immune response by promoting the clonal expansion of naive CD4
+
T-cells. It has also been shown that IL-27 has the ability to induce CD8
+
mediated tumor-specific protective immunity.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of human TF-1 cells
  • research grade: ≥ 2×
    10
    3
    U/mg
  • Selected references

    1. Batten, M. et al. (2006) Interleukin 27 limits autoimmune encephalomyelitis by suppressing the development of interleukin 17-producing T cells. Nat. Immunol. 7(9): 929-936
    2. Devergne, O. et al. (1997) Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin 12 form a novel heterodimeric hematopoietin. Proc. Natl. Acad. Sci. U.S.A. 94(22): 12041-12046
    3. Diveu, C. et al. (2009) IL-27 blocks RORc expression to inhibit lineage commitment of Tʜ17 cells. J. Immunol. 182: 5748-5756
    4. Hisada, M. et al. (2004) Potent antitumor activity of interleukin-27. Cancer Res. 64(3): 1152-1156
    5. Pflanz, S. et al. (2002)
      IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4
      +
      T cells.
      Immunity 16(6): 779-790
    6. Seita, J. et al. (2008) Interleukin-27 directly induces differentiation in hematopoietic stem cells. Blood 111(4): 1903-1912
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 2
HEK293 cells
2 µg
-
HEK293 cells
10 µg
-