Human IL-2 IS

Human IL-2 IS

Recombinant human IL-2 (interleukin 2) stimulates growth and differentiation of cells of the lymphoid lineage, such as T, NK, and B cells. IL-2 is a potent immunomodulatory cytokine, as it prevents autoimmunity and has key functions during infections. IL-2 IS stands for interleukin 2 "Improved Sequence", also termed aldesleukin, and is a variant of IL-2 with a serine substitution for the native cysteine at amino acid position 125. Human IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays, and possesses the same biological properties as naturally occurring IL-2.

Applications

Human IL-2 IS can be used for a variety of applications including:
  • In vitro activation and propagation of T cells, e.g., in combination with the T Cell Activation/Expansion Kit, human.
  • In vitro stimulation of cytolytic function and expansion of NK cells, e.g., using the NK Cell Activation/Expansion Kit, human.
  • Generation of lymphokine-activated killer (LAK) cells or cytokine-induced killer (CIK) cells.

Background information

IL-2, a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. It is produced by activated T cells, especially the CD4
+
T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- chain, and common γ-chain. The latter two are also used for IL-15 signaling.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 86/504)
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (typical activity: ≥ 9×
    10
    6
    IU/mg
    )
  • research grade: ≥ 3×
    10
    6
    IU/mg
  • Selected references

    1. Engelmann S. et al. (2013) T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J. Immunol. 191(10): 4950-4959
    2. Nguyen, T. L. et al. (2011) Antigen-specific TGF-β-induced regulatory T cells secrete chemokines, regulate T cell trafficking, and suppress ongoing autoimmunity. J. Immunol. 187(4): 1745-1753
    3. Ng, S. B. et al. (2011) Dysregulated microRNAs affect pathways and targets of biologic relevance in nasal-type natural killer/T-cell lymphoma. Blood 118(18): 4919-4929
    4. Valignat, M. P. et al. (2013) T lymphocytes orient against the direction of fluid flow during LFA-1-mediated migration. Biophys. J. 104(2): 322-331
    5. Fuchs, S. et al. (2014)
      Patients with T
      +/low
      NK
      +
      IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
      Eur. J. Immunol. 44(10): 3129-3140
    6. Pallett, L. J. et al. (2015) Metabolic regulation of hepatitis B immunopathology by myeloid-derived suppressor cells. Nat. Med. 21(6): 591-600
    7. Fallarini S. et al. (2012) Invariant NKT cells increase drug-induced osteosarcoma cell death. Br J Pharmakon 167(7): 1533-1549
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
KRW 91,600.00
E. coli
50 µg
KRW 249,800.00
E. coli
10 µg
KRW 116,600.00
E. coli
50 µg
KRW 308,000.00
E. coli
200 µg
KRW 507,800.00
E. coli
1000 µg
KRW 1,040,600.00

Related products

1 products available