The Eosinophil Isolation Kit is an indirect magnetic labeling system for the isolation of untouched eosinophils from lysed human peripheral blood.

Data and images for Eosinophil Isolation Kit, human

Figures

Figure 1

Isolation of untouched eosinophils from human peripheral blood using the Eosinophil Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells are fluorescently stained with CD16-FITC; eosinophils do not express CD16.
Unseparated fraction
Enriched eosinophils
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Figure 1

Isolation of untouched eosinophils from human peripheral blood using the Eosinophil Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells are fluorescently stained with CD16-FITC; eosinophils do not express CD16.
View details

Figure 1

Isolation of untouched eosinophils from human peripheral blood using the Eosinophil Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells are fluorescently stained with CD16-FITC; eosinophils do not express CD16.

Figure 2

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Working scheme for the isolation of untouched eosinophils using the Eosinophil Isolation Kit.

Figure 2

Working scheme for the isolation of untouched eosinophils using the Eosinophil Isolation Kit.

Specifications for Eosinophil Isolation Kit, human

Overview

The Eosinophil Isolation Kit is an indirect magnetic labeling system for the isolation of untouched eosinophils from lysed human peripheral blood.

Detailed product information

Background information

Eosinophils are involved in innate as well as adaptive immunity. They exert functions in the immunological response to various pathological conditions, e.g., parasitic infections, asthma, and gastrointestinal disorders. Eosinophils are characterized by granules that contain cytotoxic proteins (e.g. major basic protein, MBP) and ribonucleases. Eosinophils act through the release of these pre-formed molecules as well as various cytokines, chemokines, lipid mediators, and neuromodulators. They are activated in response to a number of stimuli, such as tissue injury, infections, allergens, and tumors.
Eosinophils interact with various other immune cells, e.g. by acting as antigen-presenting cells to stimulate T cells.
They also possess the capacity to activate mast cells by releasing MBP.
1

Detailed separation procedure

Non-eosinophils are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies as well as Anti-Biotin MicroBeads. Highly pure eosinophils are obtained by depletion of the magnetically labeled cells.

Downstream applications

The Eosinophil Isolation Kit can be used for the isolation of eosinophils for functional studies, studies on signal transduction, activation mechanisms, or cytokine production.

Columns

MS, LS, XS, or autoMACS
®
Columns.

References for Eosinophil Isolation Kit, human

Publications

  1. Matsuyama et al. (2007) Discoidin domain receptor 1 contributes to eosinophil survival in an NF-kappaB-dependent manner in Churg-Strauss syndrome. Blood 109: 22-30

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