The MACSelect Kk
- Transfected Cell Selection Kit contains the pMACS Kk
.II vector, a control vector, MACSelect Kk
-FITC, mouse antibody for detection of cells before enrichment, MACSelect Control FITC antibody for detection of cells after enrichment, and a CD14-FITC antibody for analysis of the control experiment when doing a co-transfection. All components are also available separately. The pMACS Kk
.II vector encodes the truncated H-2Kk
surface marker with an upstream H-2Kk
promotor, and can also be used for the expression of a gene of interest driven by a SV40 promotor. Moreover, the vector can also be used for co-transfection with an expression vector encoding the gene of interest. Transfected cells are magnetically labeled with MACSelect Kk
MicroBeads and subsequently separated from non-transfected cells.