Amplification of two identical mouse liver samples. The mRNA of two identical mouse liver tissues was isolated and cDNA was generated using the μMACS One-step T7 Template Kit in combination with the Microarray RNA Target Synthesis Kit (T7), Roche Diagnostic GmbH. Samples were labeled with Cy3 and Cy5 fluorescent dyes, respectively.
500 ng of each labeled aRNA were hybridized to PIQOR Immunology Microarrays, mouse, antisense (1076 genes). The resulting regression coefficient was 0.99.