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Flow cytometric analysis of in vitro generated monocyte-derived dendritic cells (Mo-DCs) using the Mo-DC Differentiation Inspector. Monocytes isolated with CD14 MicroBeads from human PBMCs were differentiated into Mo-DCs in the presence of GM-CSF and IL-4 for 7 days using the Mo-DC Differentiation Medium. Immature Mo-DCs were differentiated into mature Mo-DCs using the Mo-DC Maturation Medium containing TNF-α. Shown are the expression levels of CD14 (A), CD209 (DC-SIGN) (B), and CD83 (C) as mean fluorescence intensities (MFI) on monocytes, immature Mo-DCs (imMo-DCs), and mature Mo-DCs (mMo-DCs) prepared from 12 donors.
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