Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry, human

Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry, human

The Carcinoma Cell Enrichment and Detection Kit – Immunocytochemistry is a ready-to-use kit that allows for more than a 10,000-fold enrichment and the subsequent immunocytochemical detection of disseminated epithelial tumor cells from blood, bone marrow, or lymphoid tissue by the positive selection of cytokeratin 7/8 expressing cells.

Background information

Most malignant cells which have their origin in epithelial tissue express cytokeratin and can be recognized by an anti-cytokeratin antibody
1
. With the Carcinoma Cell Enrichment and Detection Kit, disseminated epithelial tumor cells can rapidly and efficiently be enriched using MACS Cytokeratin MicroBeads, e.g. from a frequency of 1 tumor cell in 10
7
leukocytes to a frequency of 1 tumor cell per 10
3
leukocytes. Thus, large patient samples can be narrowed and screened for disseminated tumor cells on only a few microscopy slides.

Detailed procedure

The carcinoma cells are permeabilized, fixed, and magnetically labeled with Anti-Cytokeratin MicroBeads. Magnetically labeled cells are then stained with a highly specific Anti-Cytokeratin-FITC antibody and Anti-FITC Alkaline Phosphatase and subjected to magnetic separation. After separation, cells are spun down onto a glass slide and stained with an alkaline phosphatase substrate.

Columns

For enrichment and in-column staining: MS Columns.
  • Selected references

    1. Heatley, M. et al. (1995) Cytokeratin intermediate filament expression in benign and malignant breast disease. J. Clin. Pathol. 48: 26-32
    2. Martin. V. M. et al. (1998) Immunomagnetic enrichment of disseminated epithelial tumor cells from peripheral blood by MACS. Exp. Hematol. 26: 252-264
    3. Gaforio, J. J. et al. (2003) Detection of breast cancer cells in the peripheral blood is positively correlated with estrogen-receptor status and predicts for poor prognosis. Int. J. Cancer 107: 984-990
    4. Campos, M. et al. (2008) Phenotypic and genetic characterization of circulating tumor cells by combining immunomagnetic selection and FICTION techniques. J. Histochem. Cytochem. 56: 667-675
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