CD69 antibodies, mouse

CD69 antibodies, mouse

Clone: H1.2F3 | Dilution: 1:10
Clone H1-2F3 recognizes mouse CD69, a type II integral membrane protein with a C-type lectin domain. CD69 is expressed as a homodimer composed of heavily glycosylated subunits. CD69 is rapidly induced upon activation of T and B cells, neutrophils, and NK cells, which is why CD69 has been mostly regarded as an activation marker. The precise role of CD69 in immunity has not been determined because its ligand is unknown. Freshly prepared thymocytes undergoing selection events express CD69, and regulatory roles for CD69 expression in T-cell development in the thymus have been suggested. However, phenotypical analysis in previous studies using CD69-deficient mice has revealed that CD69 does not appear to be required for the development of CD4 T cells. CD69 is also expressed on platelets. Recent studies have shown that CD69 is constitutively expressed by tissue-resident Th memory cells and that its function is essential for the generation of professional resting memory Th cells.

Alternative names

AIM, VEA, EA1, MLR3, gp34/28

Technical specifications

  • Antigen: CD69
  • Clone: H1.2F3
  • Isotype: hamster IgG1
  • Alternative names of antigen: AIM, VEA, EA1, MLR3, gp34/28
  • Entrez Gene ID: 12515
  • Molecular mass of antigen [kDa]: 22
  • Distribution of antigen: T cells, B cells, NK cells, neutrophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, VioGreen, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin
  • Selected references

    1. Sathaliyawala, T. et al. (2013) Distribution and compartmentalization of human circulating and tissue-resident memory T cell subsets. Immunity 38(1): 187-197
    2. Shinoda, K. et al. (2012) Type II membrane protein CD69 regulates the formation of resting T-helper memory. Proc. Natl. Acad. Sci. U.S.A. 109(19): 7409-7414
    3. Sancho, D. et al. (2005) CD69 is an immunoregulatory molecule induced following activation. Trends Immunol. 26(3): 136-140
Product options: 18
130-103-983

CD69-FITC, mouse

9 µg in 300 µL (1)
JPY 3,800
130-103-950

CD69-FITC, mouse

30 µg in 1 mL (1)
JPY 7,500
130-103-979

CD69-PE, mouse

9 µg in 300 µL (1)
JPY 4,800
130-103-946

CD69-PE, mouse

30 µg in 1 mL (1)
JPY 12,000
130-103-980

CD69-APC, mouse

9 µg in 300 µL (1)
JPY 7,000
130-103-947

CD69-APC, mouse

30 µg in 1 mL (1)
JPY 12,000
9 µg in 300 µL (1)
JPY 10,000
30 µg in 1 mL (1)
JPY 17,000
9 µg in 300 µL (1)
JPY 10,500
30 µg in 1 mL (1)
JPY 17,000
9 µg in 300 µL (1)
JPY 8,500
30 µg in 1 mL (1)
JPY 18,000
9 µg in 300 µL (1)
JPY 9,000
30 µg in 1 mL (1)
JPY 18,000
9 µg in 300 µL (1)
JPY 8,300
30 µg in 1 mL (1)
JPY 18,000
9 µg in 300 µL (1)
JPY 2,800
30 µg in 1 mL (1)
JPY 7,500