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Splenocytes from BALB/c mice, either left unstimulated (left image) or stimulated with CD3/CD28 antibodies for 3 days at 37 °C, were stained with CD223 antibodies as well as with CD3ε antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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