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A single-cell suspension of human epidermis was stained with CD207 (Langerin) antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
Flow cytometric data of FITC, PE, APC, and Biotin were kindly provided by Prof. Dr. Nikolaus Romani, Department of Dermatology & Venereology, Innsbruck Medical University, Anichstrasse 35, A-6020 Innsbruck, Austria.
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