Anti-Rb pS780 antibodies, human

Anti-Rb pS780 antibodies, human

Clone: REA433 | Dilution: 1:11
Clone REA433 recognizes the human retinoblastoma (Rb) antigen phosphorylated at serine 780 (pS780). The Rb protein is a tumor suppressor that plays a pivotal role in the negative control of the cell cycle and in tumor progression. It has been shown that Rb is responsible for a major G1 checkpoint, blocking S-phase entry, and cell growth. Loss of Rb functions may induce cell cycle deregulation, leading to a malignant phenotype. Rb negatively regulates transcription by forming complexes with members of the E2F transcription factor family. These complexes, when bound to E2F sites within certain target genes, actively repress transcription through a variety of mechanisms including physical interaction with adjacent transcriptional activation domains and recruitment of proteins that directly, or indirectly, lead to histone deacetylation. Rb function is, in turn, modulated by phosphorylation mediated by cyclin-dependent kinases. Phospho-Rb is described as being hyperphosphorylated and when in this state, it is unable to complex E2F and therefore, unable to restrict progression from the G1 phase to the S phase of the cell cycle. During the M-to-G1 transition, phospho-Rb is progressively dephosphorylated by PP1, returning to its growth-suppressive hypophosphorylated state. The pS780-Rb epitope is a robust marker of mitosis that allows cytometric detection of mitotic cells beginning with chromatin condensation and ending after cytokinesis.
Additional information: Clone REA433 displays negligible binding to Fc receptors.

Alternative names

RB1, OSRC, PPP1R130, RB, p105-Rb, pRb, pp110

Technical specifications

  • Antigen: Rb pS780
  • Clone: REA433
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: RB1, OSRC, PPP1R130, RB, p105-Rb, pRb, pp110
  • Entrez Gene ID: 5925
  • Molecular mass of antigen [kDa]: 106
  • Distribution of antigen: other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC
  • Selected references

    1. Lee, W. H. et al. (1987) The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity. Nature 329(6140): 642-645
    2. Nair, J. S. et al. (2009) Aurora B kinase regulates the postmitotic endoreduplication checkpoint via phosphorylation of the retinoblastoma protein at serine 780. Mol. Biol. Cell 20(8): 2218-2228
    3. Rubin, S. M. (2013) Deciphering the retinoblastoma protein phosphorylation code. Trends Biochem. Sci. 38(1): 12-19
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