Anti-Ly-49A/D antibodies, mouse

Anti-Ly-49A/D antibodies, mouse

Clone: REA853 | Dilution: 1:50
Clone REA853 recognizes the mouse Ly-49A and Ly-49D antigens belonging to the Ly-49 family. Members of Ly-49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands, and include activating and inhibitory receptor molecules. Ly-49A is a 44 kDa type II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains. Expression of Ly-49A is found on subset of NK and NK T cells. Ly-49D is a prototypic activating member of Ly-49 family and in contrast to inhibitory members, lacks a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). A positive signal is initiated by interaction of Ly-49D with immunoreceptor tyrosine-based activating motif (ITAM) containing transmembrane adapter DAP12.
Clone REA853 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49D.
Additional information: Clone REA853 displays negligible binding to Fc receptors.

Alternative names

Ly49A, Ly49D

Technical specifications

  • Antigen: Ly-49A/D
  • Clone: REA853
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Ly49A, Ly49D
  • Entrez Gene ID: 16627, 16635
  • Molecular mass of antigen [kDa]: 31
  • Distribution of antigen: NK cells, NKT cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, APC-Vio770, Biotin, FITC
  • Selected references

    1. Yokoyama, W. M. et al. (1993) The Ly-49 and NKR-P1 gene families encoding lectin-like receptors on natural killer cells: the NK gene complex. Annu. Rev. Immunol. 11: 613-635
    2. Mason, L. H. et al. (1996) The Ly-49D receptor activates murine natural killer cells. J. Exp. Med. 184: 2119-2128
    3. Takei, F. et al. (1997) The Ly-49 family: genes, proteins and recognition of class I MHC. Immunol. Rev. 155: 67-77
    4. Ortaldo, J. R. et al. (1998)
      The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3
      +
      cells.
      J. Immunol. 160(3): 1158-1165
    5. Ortaldo, J. R. et al. (1999) Ly-49 receptor expression and functional analysis in multiple mouse strains. J. Leukoc. Biol. 66(3): 512-520
    6. Hanke, T. et al. (1999) Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors. Immunity 11(1): 67-77
    7. Chung, D. H. et al. (2000) Mapping the ligand of the NK inhibitory receptor Ly49A on living cells. J. Immunol. 165: 6922-6932
Product options: 12
30 µg in 200 µL (1)
JPY 7,500
150 µg in 1 mL (1)
JPY 22,000
30 µg in 200 µL (1)
JPY 12,000
150 µg in 1 mL (1)
JPY 35,000
30 µg in 200 µL (1)
JPY 12,000
150 µg in 1 mL (1)
JPY 35,000
30 µg in 200 µL (1)
JPY 18,000
150 µg in 1 mL (1)
JPY 52,000
30 µg in 200 µL (1)
JPY 18,000
150 µg in 1 mL (1)
JPY 52,000
30 µg in 200 µL (1)
JPY 7,500
150 µg in 1 mL (1)
JPY 22,000

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