Anti-Granzyme B antibodies, human, mouse, rat

Anti-Granzyme B antibodies, human, mouse, rat

Clone: REA226
Clone REA226 recognizes granzyme B, a 32 kDa member of serine protease family, which is involved in cell lysis mediated by cytotoxic T lymphocytes (CTLs) and NK cells. Also known as granule enzymes, granzymes comprise majority of the mass of cytolytic granules in cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B is one of the five granzymes expressed by humans. NK cells and γδ T cells express granzymes constitutively, whereas expression in T lymphocytes is stimulation dependent. Main function attributed to granzymes is to indue apoptotic death of infected and transformed cells, where granzyme B enters into the target cell in a perforin dependent manner and via cation-independent mannose-6-phosphate receptor (CI-MPR). Granzyme B cleaves cellular substrates such as procaspase-3 and Bid, which then initiate pathways involved in apoptotic cell death. Granzyme B is suggested to be the only mammalian serine protease that prefers acidic side chains.
Additional information: Clone REA226 displays negligible binding to Fc receptors.

Alternative names

GZMB, CCPI, CGL-1, CSP-B, CTLA-1, CTSGL1, HLP, SECT, Granzyme 2

Technical specifications

  • Antigen: Granzyme B
  • Clone: REA226
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: GZMB, CCPI, CGL-1, CSP-B, CTLA-1, CTSGL1, HLP, SECT, Granzyme 2
  • Distribution of antigen: T cells, NK cells
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. Müllbacher, A. et al. (1999) Granzymes are the essential downstream effector molecules for the control of primary virus infections by cytolytic leukocytes. Proc. Natl. Acad. Sci. U.S.A. 96(13): 13950-13955
    2. Trapani, J. A. (2001) Granzymes: a family of lymphocyte granule serine proteases. Genome Biol. 2(12): 3014.1-3014.7
    3. Grossman, W. J. et al. (2004) Differential expression of granzymes A and B in human cytotoxic lymphocyte subsets and T regulatory cells. Blood 104: 2840-2848
Product options: 1
100 µg in 100 µL
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