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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Splenocytes from C57BL/6 mice were either left unstimulated (left image) or stimulated with CD3ε and CD28 antibodies as well as with interleukin 2 (IL-2) over night. Afterwards, PMA and ionomycin were added for two hours. Cells were then fixed and permeabilized using the FoxP3 Staing Buffer Set and stained with Anti-EGR-2 antibodies as well as with CD19 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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