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A431 cells, either left unstimulated (left peak) or stimulated with 100 ng/mL EGF for 5 minutes at 37 °C, were fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Anti-EGF Receptor pY1068 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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