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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left image) or stimulated with 10 ng/mL LPS and 1 µg/ml brefeldin at 37 °C for 24 hours. Cells were then fixed, permeabilize,d and stained with Anti-CCL2 (MCP-1) antibodies as well as with CD14 antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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