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EA.hy926 cells, overnight serum starved and stimulated with 150 nM Calyculin A for 30 minutes, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were stained with REA Control (I) antibodies or with the corresponding Anti-p120 Catenin (pT916) antibodies (right peaks). In case of Vio®
515, Jurkat cells were fixed, permeabilized, and stained with REA Control (I) antibodies or with the corresponding Anti-Ki-67 antibodies (right peaks). Cells were analyzed by flow cytometry using the MACSQuant®
Cell debris were excluded from the analysis based on scatter signals.
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