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MZ and FO B cells were isolated from mouse spleen cell suspension by using the Marginal Zone and Follicular B Cell Isolation Kit, mouse. The cells were fluorescently stained with CD45R (B220)- VioBlue (# 130-094-287), CD21/CD35-PE-Vio770 (# 130-097-216), CD23-PE (# 130-097-819), and CD93 (AA4.1)-APC and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Flow-through fraction: unlabeled enriched MZ B cells
Eluted fraction: enriched FO B cells
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