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A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD19+
B cells were isolated from this single-cell suspension using the CD19 MicroBeads, and autoMACS®
Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45R (B220)-APC-Vio®
770 (clone REA755), mouse, and CD19-PE-Vio770, mouse (clone REA749) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals and 7-AAD Staining Solution fluorescence.
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