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Detection and isolation of viable BKV–specific T cells using MACS® IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE)
From a BKV+
human peripheral blood mononuclear cells (PBMCs) were restimulated for 4 hours either with 20 μL/mL of reconstituted PepTivator®
BKV LT1 (A), 20 µL/mL BKV VP1 (B), or a mixture of both peptide pools (C). BKV-specific T cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay – Cell Enrichment and Detection Kit (PE) (# 130-054-201). T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. IFN-γ secretion of viable lymphocytes is shown.
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