Name: Human SHH (C24II)
Availability: InStock

Product data and images for Human SHH (C24II)

Figures

Figure 1

Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.

TH (tyrosine hydroxylase) ⁺ cells	TuJ1 (neuron-specific β-III tubulin) ⁺ cells	GAD67 (glutamate decarboxylase) ⁺ cells
View details Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.	View details Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.	View details Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.

Figure 2

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Figure 2

Human SHH (C24II) activity assay.

The biological activity of Human SHH (C24II) is determined by alkaline phosphatase assay using mouse C3H10T1/2 fibroblasts.

Human SHH (C24II) activity assay.

The biological activity of Human SHH (C24II) is determined by alkaline phosphatase assay using mouse C3H10T1/2 fibroblasts.

Figure 3

View details

Figure 3

SDS-PAGE of Human SHH (C24II), premium grade

under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human SHH (C24II), premium grade

under reduced (R) and non-reduced (NR) conditions.

Figure 4

View details

Figure 4

Mass spectrometry analysis (ESI-MS) of Human SHH (C24II), premium grade. The peak corresponds to the calculated molecular mass of 19683 Da.

Specifications for Human SHH (C24II)

Overview

Recombinant Human SHH (Sonic Hedgehog; or C24II) can promote growth and differentiation of embryonic stem cells, adult mesenchymal stem cells, and cells within the neuronal lineage. The morphogenetic cytokine is crucial for embryonic development, as it is involved in the patterning of the central nervous system, somites, and limbs. Recombinant Human SHH is ideal for use in cell culture, differentiation studies, and functional assays.

Applications

Human SHH (C24II) can be used for a variety of applications including:

Expansion or differentiation of various cell types, mainly human and mouse embryonic stem cells, but also adult stem cells and mesenchymal cells.

Detailed product information

Background information

Sonic hedgehog (SHH) is involved in a wide variety of embryonic developmental events, including neurogenesis, limb development, hematopoiesis, and gut formation. Besides their role as morphogens, hedgehog family members have also been shown to act as mitogens, cell survival factors, and axon guidance factors and to influence tissue regeneration within the adult. SHH is synthesized as a precursor, which is autocatalytically cleaved into a 19 kDa N-terminal domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N is responsible for the signaling activity of hedgehog proteins, while SHH-C acts as a cholesterol transferase and covalently attaches a cholesterol molecule to SHH-N. The lipid-modified SHH-N displays enhanced activity. Human SHH (C24II) corresponds to the mature SHH-N domain and carries a Cys to Ile-Ile substitution resulting in a lipophilic moiety and therefore an increased activity.

Biological activity

Alkaline phosphatase production by mouse C3H10T1/2 fibroblasts
premium grade: ≥ 2×
10
³
U/mg
research grade: ≥ 1×
10
³
U/mg

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Resources for Human SHH (C24II)

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for Human SHH (C24II)

Publications

Nakamura, T. et al. (1997) Induction of osteogenic differentiation by hedgehog proteins. Biochem. Biophys. Res. Commun. 237: 465-469

Data and images

Data and images for Human SHH (C24II)

Figures

Figure 1

TH (tyrosine hydroxylase) ⁺ cells	TuJ1 (neuron-specific β-III tubulin) ⁺ cells	GAD67 (glutamate decarboxylase) ⁺ cells
View details Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.	View details Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.	View details Figure 1 Neural precursors, derived from mouse embryonic stem cells, were expanded in MACS Neuro Medium containing N2-supplements, laminin, Human FGF-2 (10 ng/mL), Human SHH (C24II) (200 ng/mL), and FGF-8b (100 ng/mL). Subsequently cells were grown in MACS Neuro Medium complemented by MACS NeuroBrew-21 and ascorbic acid (200 µM) for final differentiation into neurons. Cells were analyzed by immunocytochemistry for TuJ1, TH, and GAD67 expression.

Figure 2

View details

Figure 2

Human SHH (C24II) activity assay.

The biological activity of Human SHH (C24II) is determined by alkaline phosphatase assay using mouse C3H10T1/2 fibroblasts.

Human SHH (C24II) activity assay.

The biological activity of Human SHH (C24II) is determined by alkaline phosphatase assay using mouse C3H10T1/2 fibroblasts.

Figure 3

View details

Figure 3

SDS-PAGE of Human SHH (C24II), premium grade

under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human SHH (C24II), premium grade

under reduced (R) and non-reduced (NR) conditions.

Figure 4

View details

Figure 4

Mass spectrometry analysis (ESI-MS) of Human SHH (C24II), premium grade. The peak corresponds to the calculated molecular mass of 19683 Da.

Specifications

Specifications for Human SHH (C24II)

Overview

Applications

Human SHH (C24II) can be used for a variety of applications including:

Expansion or differentiation of various cell types, mainly human and mouse embryonic stem cells, but also adult stem cells and mesenchymal cells.

Detailed product information

Background information

Biological activity

Alkaline phosphatase production by mouse C3H10T1/2 fibroblasts
premium grade: ≥ 2×
10
³
U/mg
research grade: ≥ 1×
10
³
U/mg

Quality description

Research-grade

Premium-grade

Resources

Resources for Human SHH (C24II)

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References

References for Human SHH (C24II)

Publications

Nakamura, T. et al. (1997) Induction of osteogenic differentiation by hedgehog proteins. Biochem. Biophys. Res. Commun. 237: 465-469

Human SHH (C24II)

Human SHH (C24II)

Product overview

Product data and images for Human SHH (C24II)

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Specifications for Human SHH (C24II)

Overview

Applications

Detailed product information

Background information

Biological activity

Quality description

Resources for Human SHH (C24II)

Certificates

References for Human SHH (C24II)

Publications

Data and images for Human SHH (C24II)

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Specifications for Human SHH (C24II)

Overview

Applications

Detailed product information

Background information

Biological activity

Quality description

Resources for Human SHH (C24II)

Certificates

References for Human SHH (C24II)

Publications

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