CD25 antibodies

CD25 antibodies

Clone: REA570
Clone REA570 recognizes the human CD25 glycoprotein, also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). It associates with IL-2 receptor β (CD122) and γ chains (CD132) to form a high affinity IL-2R complex. CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4
+
regulatory T cells. Clone REA570 recognizes epitope region A.
Additional information: Clone REA570 displays negligible binding to Fc receptors

Alternative names

IL-2Rα, p55, Tac

Technical specifications

  • Antigen: CD25
  • Clone: REA570
  • Isotype: recombinant human IgG1
  • Alternative names of antigen: IL-2Rα, p55, Tac
  • Molecular mass of antigen [kDa]: 28
  • Distribution of antigen: B cells, basophils, lymphocytes, macrophages, monocytes, myeloid cells, NK cells, osteoblasts, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C (36–46 °F). Do not freeze.
  • Available conjugates: APC
  • Selected references

    1. Lee, S. H. et al. (2012) Cutting edge: a novel mechanism bridging innate and adaptive immunity: IL-12 induction of CD25 to form high-affinity IL-2 receptors on NK cells. J. Immunol. 189(6): 2712-2716
    2. Piccirillo, C. A. et al. (2006)
      Development and function of naturally occurring CD4
      +
      CD25
      +
      regulatory T cells.
      J. Leukoc. Biol. 3: 458-470
    3. Goudy, K. et al. (2013) Human IL2RA null mutation mediates immunodeficiency with lymphoproliferation and autoimmunity. Clin. Immunol. 146(3): 248-261
    4. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    5. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    6. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    7. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    8. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 1
170-081-060

CD25-APC

1 mL
JPY 88,000