Clone:
REA851
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Transmembrane 4 L6 family member 1

Extended validation for TM4SF1 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of TM4SF1 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TM4SF1-PE (REA851, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of TM4SF1 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TM4SF1-PE (REA851, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of TM4SF1 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with TM4SF1-PE (REA851, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of TM4SF1 knockout cells. Wild type (red) and knockout cells (blue) were stained with TM4SF1-PE, Clone (REA851). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of TM4SF1 knockout cells. Wild type (red) and knockout cells (blue) were stained with TM4SF1-PE, Clone (REA851). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for TM4SF1 Antibody, anti-human, REAfinity™

Overview

Clone REA851 recognizes the human TM4SF1 antigen, also known as transmembrane 4 L6 family member 1. TM4SF1 is a member of the tetraspanin family, which members are involved in signal transduction events, important for the regulation of cell development, activation, growth, and motility. It is reported, that TM4SF1 suppresses the expression of CD63 and CD82, two molecules that inhibit cell mobility. TM4SF1 is highly expressed in lung, breast, colon, and ovarian carcinomas. It is also found on some endothelial cells.
Additional information: Clone REA851 displays negligible binding to Fc receptors.

Alternative names

Transmembrane 4 L6 family member 1

Detailed product information

Technical specifications

CloneREA851
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenTM4SF1
Alternative names of antigenTransmembrane 4 L6 family member 1
Molecular mass of antigen [kDa]22
Distribution of antigenendothelial cells, lung cancer cells, breast cancer cells, colorectal carcinoma cells
Entrez Gene ID4071
RRIDAB_2654199, AB_2654200, AB_2654201, AB_2654202, AB_2654203, AB_2654204, AB_2654205, AB_2654198

Resources for TM4SF1 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for TM4SF1 Antibody, anti-human, REAfinity™

Publications

  1. Marken, J. S. et al. (1992) Cloning and expression of the tumor-associated antigen L6. Proc. Natl. Acad. Sci. U.S.A. 89(8): 3503-3507
  2. Marken, J. S. et al. (1994) Membrane topology of the L6 antigen and identification of the protein epitope recognized by the L6 monoclonal antibody. J. Biol. Chem. 269(10): 7397-7401

Related products for
TM4SF1 Antibody, anti-human, REAfinity™

1 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?