Clone:
REA847
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Ly-76, Ly76

Extended validation for Ter-119 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA847
TER-119++
Cells were incubated with an excess of purified unconjugated Ter-119 (REA847) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Ter-119. Mouse splenocytes were stained with Ter-119 antibodies and with a suitable counterstaining. As a control, Ter-119 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using Ter-119 (REA847). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using Ter-119 (REA847). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using Ter-119 (REA847). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Ter-119 Antibody, anti-mouse, REAfinity™

Overview

Clone REA847 recognizes the mouse Ter-119 antigen, a glycophorin A–associated protein, also known as Ly-76. Ter-119 is expressed on mature erythrocytes and erythroid precursor cells in adult blood, spleen, and bone marrow, as well as in the embryonic yolk sac and fetal liver. REA847 does not react with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activity. In adult mice, REA847 reatcts with 20–25% of bone marrow cells and approximately 50% of spleen cells, but not with thymocytes or lymph node cells.
Additional information: Clone REA847 displays negligible binding to Fc receptors.

Alternative names

Ly-76, Ly76

Detailed product information

Technical specifications

CloneREA847
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenTer-119
Alternative names of antigenLy-76, Ly76
Distribution of antigenred blood cells, bone marrow, spleen
Entrez Gene ID104231
RRIDAB_2654114, AB_2654115, AB_2654116, AB_2654117, AB_2654118, AB_2654119, AB_2654120, AB_2654121, AB_2654122, AB_2654123, AB_2654124, AB_2654125, AB_2654126, AB_2654127, AB_2654128, AB_2654129, AB_2654130, AB_2654113

Resources for Ter-119 Antibody, anti-mouse, REAfinity™

Certificates

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to search for Certificates of Analysis (CoA) by lot number.

References for Ter-119 Antibody, anti-mouse, REAfinity™

Publications

  1. Vannucchi, A. M. et al. (2000) Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice. Blood 95(8): 2559-2568

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