Clone:
REA318
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
TCRB, TCRbeta, Tib

Extended validation for TCRβ Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA318
H57-597++
Cells were incubated with an excess of purified unconjugated TCRβ (REA318) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for TCRβ. Splenocytes from C57BL/6 mice were stained with TCRβ antibodies and with a suitable counterstaining. As a control, TCRβ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCRβ. Splenocytes from C57BL/6 mice were stained with TCRβ antibodies and with a suitable counterstaining. As a control, TCRβ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for TCRβ. Splenocytes from C57BL/6 mice were stained with TCRβ antibodies and with a suitable counterstaining. As a control, TCRβ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for TCRβ. Splenocytes from C57BL/6 mice were stained with TCRβ antibodies and with a suitable counterstaining. As a control, TCRβ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRβ (REA318). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TCRβ (REA318). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TCRβ (REA318). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCRβ Antibody, anti-mouse, REAfinity™

Overview

Clone REA318 recognizes the mouse T cell receptor beta chain (TCRβ). The T cell receptor (TCR) is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a disulfide-linked membrane-anchored heterodimeric glycoprotein normally consisting of the highly variable alpha and beta chains expressed as part of a complex with the invariant CD3 chain molecules. T cells expressing this receptor are referred to as TCRα/β T cells, though a minority of T cells express an alternate receptor, formed by variable gamma and delta chains, referred as TCRγ/δ T cells. TCRβ is expressed on TCRα/β T cells and thymocytes, NK1.1
+
thymocytes and NK-T cells.
Additional information: Clone REA318 displays negligible binding to Fc receptors.

Alternative names

TCRB, TCRbeta, Tib

Detailed product information

Technical specifications

CloneREA318
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenTCRβ
Alternative names of antigenTCRB, TCRbeta, Tib
Distribution of antigenT cells
Entrez Gene ID21577
RRIDAB_2752042, AB_2801791, AB_2857569, AB_2654023, AB_2654024, AB_2752053

Resources for TCRβ Antibody, anti-mouse, REAfinity™

References for TCRβ Antibody, anti-mouse, REAfinity™

Publications

  1. Robins, H.S. et al. (2009) Comprehensive assessment of T-cell receptor β-chain diversity in αβ T cells. Blood 114(19): 4099-4107
  2. Schumm, M. et al. (2013) Depletion of T-cell receptor alpha/beta and CD19 positive cells from apheresis products with the CliniMACS device. Cytotherapy 15(10): 1253-1258
  3. Okuno, Y. et al. (2013)
    CD8
    +
    CD122
    +
    regulatory T cells contain clonally expanded cells with identical CDR3 sequences of the T-cell receptor β-chain.
    Immunology 139(3): 309-317

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