T Cell TransAct™, human

Name: T Cell TransAct™, human
Availability: InStock

T Cell TransAct™, human

T cell activation via CD3 and CD28 with T Cell TransAct™. This stimulation reagent is ready-to-use for

in vitro

activation and expansion of human T cells. Its polymeric nanomatrix structure ensures gentle and efficient activation of resting T cells from PBMCs and of enriched T cell populations, while maintaining high viability.

Product data and images for T Cell TransAct™, human

Figures

Figure 1

Comparison of T cell expansion after stimulation with T Cell TransAct and TexMACS™ Medium (serum-free) or a bead-based competitor product and RPMI (10% serum) was identical. Both media were supplemented with human IL-2 IS, Premium-Grade (50 U/mL).

Figure 1

Comparison of T cell expansion after stimulation with T Cell TransAct and TexMACS™ Medium (serum-free) or a bead-based competitor product and RPMI (10% serum) was identical. Both media were supplemented with human IL-2 IS, Premium-Grade (50 U/mL).

Figure 2

Human purified T cells were isolated using the Pan T Cell Isolation Kit and activated for 48 hours using the T Cell TransAct™ (titer 1:100) in TexMACS™ Medium supplemented with Human IL-2 (20 IU/mL). The negative control experiment was performed without adding the T Cell TransAct. Cells were fluorescently stained using CD25-PE and CD69-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. CD4-VioBlue

^®

was used for selection of T helper cells and CD8-VioGreen™ was used for selection of cytotoxic T cells. Dead cells and debris were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Negative control

CD4 ⁺ T cells	CD8 ⁺ T cells
View details T Cell TransAct™, human Figure 2 Human purified T cells were isolated using the Pan T Cell Isolation Kit and activated for 48 hours using the T Cell TransAct™ (titer 1:100) in TexMACS™ Medium supplemented with Human IL-2 (20 IU/mL). The negative control experiment was performed without adding the T Cell TransAct. Cells were fluorescently stained using CD25-PE and CD69-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. CD4-VioBlue ^® was used for selection of T helper cells and CD8-VioGreen™ was used for selection of cytotoxic T cells. Dead cells and debris were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details T Cell TransAct™, human Figure 2 Human purified T cells were isolated using the Pan T Cell Isolation Kit and activated for 48 hours using the T Cell TransAct™ (titer 1:100) in TexMACS™ Medium supplemented with Human IL-2 (20 IU/mL). The negative control experiment was performed without adding the T Cell TransAct. Cells were fluorescently stained using CD25-PE and CD69-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. CD4-VioBlue ^® was used for selection of T helper cells and CD8-VioGreen™ was used for selection of cytotoxic T cells. Dead cells and debris were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
T cells after activation

CD4

⁺

T cells

CD8

⁺

cells

Figure 2

Human purified T cells were isolated using the Pan T Cell Isolation Kit and activated for 48 hours using the T Cell TransAct™ (titer 1:100) in TexMACS™ Medium supplemented with Human IL-2 (20 IU/mL). The negative control experiment was performed without adding the T Cell TransAct. Cells were fluorescently stained using CD25-PE and CD69-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. CD4-VioBlue

^®

was used for selection of T helper cells and CD8-VioGreen™ was used for selection of cytotoxic T cells. Dead cells and debris were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 2

Human purified T cells were isolated using the Pan T Cell Isolation Kit and activated for 48 hours using the T Cell TransAct™ (titer 1:100) in TexMACS™ Medium supplemented with Human IL-2 (20 IU/mL). The negative control experiment was performed without adding the T Cell TransAct. Cells were fluorescently stained using CD25-PE and CD69-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. CD4-VioBlue

^®

was used for selection of T helper cells and CD8-VioGreen™ was used for selection of cytotoxic T cells. Dead cells and debris were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for T Cell TransAct™, human

Overview

T cell activation via CD3 and CD28 with T Cell TransAct™. This stimulation reagent is ready-to-use for

in vitro

activation and expansion of human T cells. Its polymeric nanomatrix structure ensures gentle and efficient activation of resting T cells from PBMCs and of enriched T cell populations, while maintaining high viability.

Detailed product information

Background information

T Cell TransAct™ consists of a colloidal polymeric nanomatrix conjugated to humanized CD3 and CD28 agonists providing primary and co-stimulatory signals for an optimized and efficient T cell activation and expansion. The nanoscale structure of T Cell TransAct™ allows sterile filtration and excess reagent can be removed by centrifugation with following conventional supernatant replacement or simply by medium wash.

Polyclonal T cell expansion can be used when increased numbers of effector cells are required without the use of feeder cells or antigens, or when T cells are activated to enable gene modification.

Applications

In vitro

stimulation and expansion of T cell populations, either from purified T cells or from PBMCs.

Resources for T Cell TransAct™, human

Documents and Protocols

Brochures/posters

T Cell TransAct - Efficient T cell activation and expansion

Brochures/posters

Get your CAR T cell research moving

Brochures/posters

CAR T cell research and discovery

Scientific posters

Polyclonal T cell activation and expansion tool for clinical-scale manufacturing of gene-modified T cells

App notes/customer reports

Fast and efficient T cell isolation without density gradient centrifugation for CAR T cell engineering

App notes/customer reports

Generation of functional CAR T cells from pan T cells isolated by MACS® technology

App notes/customer reports

Translatability of T Cell TransAct™ for T cell activation process development

App notes/customer reports

Generation of functional CAR T cells from CD4⁺ and CD8⁺ T cells

Application protocol

Engineering of CAR T cells for research use

References

T Cell TransAct™ – Next-generation T cell activation and expansion reagent - selected references

Data and images