Name: T Cell Activation/Expansion Kit, human
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Product data and images for T Cell Activation/Expansion Kit, human

Figures

Figure 1

Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.

Stimulated sample	Unstimulated control
View details Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.	View details Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.
Stimulated sample	Unstimulated control
View details Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.	View details Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.

Specifications for T Cell Activation/Expansion Kit, human

Overview

The T Cell Activation/Expansion Kit was developed to activate and expand human T cells. The kit consists of Anti-Biotin MACSiBead Particles and biotinylated antibodies against human CD2, CD3, and CD28. Anti-Biotin MACSiBead Particles loaded with the biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells from PBMCs as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 14 of culture.

Detailed product information

Downstream applications

Activated T cells can be used for any downstream processing, such as cytokine analysis

or immunoprecipitation, and cells can also be transfected with high efficiency. The kit is suitable for the expansion of antigen-specific T cell lines and clones. It is also suitable for the activation of human CD3

⁺

CD56

⁺

NKT cells.

References for T Cell Activation/Expansion Kit, human

Publications

Lécureuil et al. (2007) Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood 109: 3649-3657
Acosta-Rodriguez, E. V. et al. (2007) Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol. 8: 942-948
Sattler et al. (2009) Cytokine-induced human IFN-gamma-secreting effector-memory Th cells in chronic autoimmune inflammation. Blood 113: 1948-1956
Diveu, C. et al. (2009) IL-27 blocks RORc expression to inhibit lineage commitment of Th17 cells. J. Immunol. 182: 5748-5756
Camargo, J. F. et al. (2009) CCR5 expression levels influence NFAT translocation, IL-2 production, and subsequent signaling events during T lymphocyte activation. J. Immunol. 182: 171-182
Duraisingham, S. S. et al. (2009) TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived dendritic cells fail to induce Tʜ17 polarization of naive T cells but do stimulate Tʜ1 and Tʜ17 memory responses. J. Immunol. 183: 2242-2251
Wieckowski, E. U. et al. (2009)
Tumor-derived microvesicles promote regulatory T cell expansion and induce apoptosis in tumor-reactive activated CD8
⁺
T lymphocytes.
J. Immunol. 183: 3720-3730

Data and images

Data and images for T Cell Activation/Expansion Kit, human

Figures

Figure 1

Stimulated sample	Unstimulated control
View details Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.	View details Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.
Stimulated sample	Unstimulated control
View details Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.	View details Figure 1 Anti-Biotin MACSiBead Particles were loaded with CD2, CD3, and CD28 antibodies. T cells were isolated using the Pan T Cell Isolation Kit. Purified T cells were activated for 72 hours using one loaded Anti-Biotin MACSiBead Particle per two T cells. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4-FITC and CD25-PE or CD4-PE and CD69-FITC.

Specifications

Specifications for T Cell Activation/Expansion Kit, human

Overview

Detailed product information

Downstream applications

Activated T cells can be used for any downstream processing, such as cytokine analysis

⁺

CD56

⁺

NKT cells.

References

References for T Cell Activation/Expansion Kit, human

Publications

Lécureuil et al. (2007) Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients. Blood 109: 3649-3657
Acosta-Rodriguez, E. V. et al. (2007) Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol. 8: 942-948
Sattler et al. (2009) Cytokine-induced human IFN-gamma-secreting effector-memory Th cells in chronic autoimmune inflammation. Blood 113: 1948-1956
Diveu, C. et al. (2009) IL-27 blocks RORc expression to inhibit lineage commitment of Th17 cells. J. Immunol. 182: 5748-5756
Camargo, J. F. et al. (2009) CCR5 expression levels influence NFAT translocation, IL-2 production, and subsequent signaling events during T lymphocyte activation. J. Immunol. 182: 171-182
Duraisingham, S. S. et al. (2009) TLR-stimulated CD34 stem cell-derived human skin-like and monocyte-derived dendritic cells fail to induce Tʜ17 polarization of naive T cells but do stimulate Tʜ1 and Tʜ17 memory responses. J. Immunol. 183: 2242-2251
Wieckowski, E. U. et al. (2009)
Tumor-derived microvesicles promote regulatory T cell expansion and induce apoptosis in tumor-reactive activated CD8
⁺
T lymphocytes.
J. Immunol. 183: 3720-3730

T Cell Activation/Expansion Kit, human

T Cell Activation/Expansion Kit, human

Product overview

Product data and images for T Cell Activation/Expansion Kit, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for T Cell Activation/Expansion Kit, human

Overview

Detailed product information

Downstream applications

References for T Cell Activation/Expansion Kit, human

Publications

Data and images

Data and images for T Cell Activation/Expansion Kit, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications

Specifications for T Cell Activation/Expansion Kit, human

Overview

Detailed product information

Downstream applications

References

References for T Cell Activation/Expansion Kit, human

Publications

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T Cell Activation/Expansion Kit, human

T Cell Activation/Expansion Kit, human

Product overview

Product data and images for T Cell Activation/Expansion Kit, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for T Cell Activation/Expansion Kit, human

Overview

Detailed product information

Downstream applications

References for T Cell Activation/Expansion Kit, human

Publications

Data and images for T Cell Activation/Expansion Kit, human

Figures

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Specifications for T Cell Activation/Expansion Kit, human

Overview

Detailed product information

Downstream applications

References for T Cell Activation/Expansion Kit, human

Publications

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