Clone:
REA459
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
BIRC5, API4, EPR-1, IAP4

Extended validation for Survivin Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA459
91630++
Cells were incubated with an excess of purified unconjugated Survivin (REA459) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for Survivin Antibody, anti-human, REAfinity™

Overview

Clone REA459 recognizes the human survivin antigen, a member of the inhibitor of apoptosis (IAP) family. Survivin is also known as baculoviral IAP repeat-containing protein 5 (BIRC5) or apoptosis inhibitor 4 (API4). The survivin protein inhibits caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. Expression of survivin is highly regulated by the cell cycle and is only expressed in the G2-M phase. Survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. It is expressed highly in most human tumors and fetal tissue, but is completely absent in terminally differentiated cells. Its overexpression has been demonstrated in breast and lung cancer, prostate, gastric, colon, bladder and esophageal carcinomas, osteosarcomas, and lymphomas. Overexpression of survivin was also found to be significantly associated with a poor prognosis and decreased survival rates in oral, breast, and colorectal cancers.
Additional information: Clone REA459 displays negligible binding to Fc receptors.

Alternative names

BIRC5, API4, EPR-1, IAP4

Detailed product information

Technical specifications

CloneREA459
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenSurvivin
Alternative names of antigenBIRC5, API4, EPR-1, IAP4
Molecular mass of antigen [kDa]16
Distribution of antigenbreast cancer cells, lung cancer cells, prostate cancer cells, bladder, colon
Entrez Gene ID332
RRIDAB_2733895, AB_2653604, AB_2653605, AB_2653608, AB_2653609, AB_2733894

Resources for Survivin Antibody, anti-human, REAfinity™

References for Survivin Antibody, anti-human, REAfinity™

Publications

  1. Ambrosini, G. et al. (1997) A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 3(8): 917-921
  2. Cheung, C. H. et al. (2013) Survivin - biology and potential as a therapeutic target in oncology. Onco Targets Ther. 16(6): 1453-1462
  3. Fukuda, S. et al. (2006) Survivin, a cancer target with an emerging role in normal adult tissues. Mol. Cancer Ther. 5(5): 1087-1098

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