Name: Recombinant SARS-CoV-2 Spike-S2 (insect cells)
Availability: InStock

Product data and images for Recombinant SARS-CoV-2 Spike-S2 (insect cells)

Figures

Figure 1

**Bead-based immunoassay for detection of patient-derived SARS-CoV-2 antibodies**
. Schematic overview illustrating how our biotinylated antigens can be applied to detect patient-derived SARS-CoV-2 antibodies

Figure 1

Bead-based immunoassay for detection of patient-derived SARS-CoV-2 antibodies

. Schematic overview illustrating how our biotinylated antigens can be applied to detect patient-derived SARS-CoV-2 antibodies

Figure 2

**Results of the bead-based immunoassay using biotinylated**
Recombinant SARS-CoV-2 Spike-S2 (insect cells). The amount of captured SARS-CoV-2 antibodies was quantified at different plasma dilutions. Results for plasma from a COVID-19
⁺
patient (circle) or a healthy donor (square) are shown.

Figure 2

Results of the bead-based immunoassay using biotinylated

Recombinant SARS-CoV-2 Spike-S2 (insect cells). The amount of captured SARS-CoV-2 antibodies was quantified at different plasma dilutions. Results for plasma from a COVID-19

⁺

patient (circle) or a healthy donor (square) are shown.

Figure 3

**ELISA of Recombinant SARS-CoV-2 Spike-S2 (insect cells).**
Recombinant SARS-CoV-2 Spike-S2 (insect cells)-Biotin can be detected by ELISA assays. Quantification at different protein concentrations illustrates that Miltenyi Biotec's recombinant S2 protein is recognized by anti-S2 antibodies.

Figure 3

ELISA of Recombinant SARS-CoV-2 Spike-S2 (insect cells).

Recombinant SARS-CoV-2 Spike-S2 (insect cells)-Biotin can be detected by ELISA assays. Quantification at different protein concentrations illustrates that Miltenyi Biotec's recombinant S2 protein is recognized by anti-S2 antibodies.

Figure 4

**SDS-PAGE of biotinylated Recombinant SARS-CoV-2 Spike-S2**
(insect cells) under reduced (R) and non-reduced (NR) conditions.

Figure 4

SDS-PAGE of biotinylated Recombinant SARS-CoV-2 Spike-S2

(insect cells) under reduced (R) and non-reduced (NR) conditions.

Figure 5

**Mass spectrometry analysis (ESI-MS) of Recombinant SARS-CoV-2 Spike-S2 (insect cells).**
The non-biotinylated protein was analyzed and the peak corresponds to the deglycosylated molecular mass (61233 Da).

Figure 5

Mass spectrometry analysis (ESI-MS) of Recombinant SARS-CoV-2 Spike-S2 (insect cells).

The non-biotinylated protein was analyzed and the peak corresponds to the deglycosylated molecular mass (61233 Da).

Specifications for Recombinant SARS-CoV-2 Spike-S2 (insect cells)

Overview

The SARS-CoV-2 S2 protein (Spike glycoprotein subunit 2) is localized at the C-terminus of the Spike protein and mediates membrane penetration and virus–cell fusion through the presence of so called heptad repeats. The SARS-CoV-2 Spike-S2 (insect cells) protein covers amino acids S686 to K1211 and contains the stabilizing proline substitutions at position K986P and V987P. The protein is extended at its C-terminus with a His-tag and an AviTag

^TM

Resources for Recombinant SARS-CoV-2 Spike-S2 (insect cells)

Documents and Protocols

Scientific posters

Detection of Humoral and Cellular Responses to SARS-CoV-2 in COVID-19 Convalescents

Application protocol

Bead-based immunoassay for detection of patient-derived SARS-CoV-2 antibodies

Data and images