PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™
PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™
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Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | |
Staining 1 |
 View details PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | |
Staining 2 | |
 View details PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |  View details PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
 View details PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | |
Staining 1 |
View details PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | |
Staining 2 | |
View details PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. | View details PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
View details PSC-mDA Neuron Analysis Kit, anti-human, REAfinity™Figure 2Example of immunofluorescent staining and analysis using the PSC-mDA Neuron Phenotyping Kit, human: Combination of the markers included in the PSC-mDA Neurons Phenotyping Kit, human (FoxA2, OTX2, PAX-6, TTF-1, Sox1, and Oct3/4) allows to determine the identity and purity of the cell product in a flow cytometry assay. iPSC-derived mDA neurons were generated and analyzed after 16 days of differentiation. For the analysis, gates were defined using cellular controls. Staining 1 shows no measurable contamination of residual Oct3/4 + pluripotent stem cells. Staining 2 shows the purity of the target mDA neurons (FoxA2 +OTX2 +) as well as contamination by other cell populations. Specifically, the PAX-6-specific antibody detects cells of a dorsal phenotype, whereas a caudal phenotype is indicated by loss of OTX2 expression. Finally, staining 3 shows expression levels of SOX-1, a neural ectoderm marker that identifies contamination of different neural cell populations, and expression of TTF-1, which is a ventral forebrain marker but can be partially expressed by the target cells. |
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