Clone:
REA899
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
6M21, Ly89, GP91, LIR-3, LILRB3

Extended validation for PIR-B Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA899
326414 (PIR-B)++
6C1 (PIR-A/B)-
10-1-PIR (PIR-A/B)++
404127 (PIR-A/B rIgG2a)+
REA472-
Cells were incubated with an excess of purified unconjugated PIR-B (REA899) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using PIR-B (REA899). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using PIR-B (REA899). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using PIR-B (REA899). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for PIR-B Antibody, anti-mouse, REAfinity™

Overview

Clone REA899 recognizes the B isoform of the mouse paired immunoglobulin-like receptor (PIR-A and PIR-B) antigen, a single-pass type I membrane protein which is also known as leukocyte immunoglobulin-like receptor 3 (LIR-3). Cell surface expression of PIR molecules on myeloid and B lineage cells increase with cellular differentiation and activation. PIR molecules are also expressed on dendritic cells, monocytes/macrophages, and mast cells in varying levels, but not on T cells and NK cells. PIRs functions as receptors for class I MHC antigens. PIR-B down-regulates antigen-induced B cell–activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).
Additional information: Clone REA899 displays negligible binding to Fc receptors.

Alternative names

6M21, Ly89, GP91, LIR-3, LILRB3

Detailed product information

Technical specifications

CloneREA899
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenPIR-B
Alternative names of antigen6M21, Ly89, GP91, LIR-3, LILRB3
Molecular mass of antigen [kDa]91
Distribution of antigenB cells, macrophages, mast cells, monocytes
Entrez Gene ID18733
RRIDAB_2726792, AB_2726824, AB_2726793, AB_2726825, AB_2726822, AB_2726791, AB_2726823

Resources for PIR-B Antibody, anti-mouse, REAfinity™

References for PIR-B Antibody, anti-mouse, REAfinity™

Publications

  1. Kubagawa, H. et al. (1997) A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells. Proc. Natl. Acad. Sci. U.S.A. 94(10): 5261-5266
  2. Bléry, M. et al. (1998) The paired Ig-like receptor PIR-B is an inhibitory receptor that recruits the protein-tyrosine phosphatase SHP-1. Proc. Natl. Acad. Sci. U.S.A. 95(5): 2446-2451
  3. Kubagawa, H. et al. (1999) Biochemical nature and cellular distribution of the paired immunoglobulin-like receptors, PIR-A and PIR-B. J. Exp. Med. 189(2): 309-318

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