Perforin Antibody, anti-human, REAfinity™

Name: Perforin Antibody, anti-human, REAfinity™
Availability: InStock

Perforin Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA1061

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

ICFC, MC

Alternative names:

PRF1, FLH2, HPLH2, P1, PFN1, PFP

Intracellular flow cytometry applications forPerforin Antibody, anti-human, REAfinity™

APC
APC-Vio 770
FITC
PE
PE-Vio 770
Vio B515
Vio R667
VioGreen

Conjugate:

APC

Recommended dilution:

1:50

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-118-191, 130-118-118

Protocols:

Intracellular flow cytometry staining protocol (Inside Stain Kit 1:50)

Description:

Fixed and permeabilized human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Perforin antibodies or with the corresponding REA Control (I) antibodies (left images) as well as with CD8 antibodies. Cells were analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris were excluded from the analysis based on scatter signals.

Mass cytometry applications forPerforin Antibody, anti-human, REAfinity™

pure

Conjugate:

pure

Products used:

130-126-480

Extended validation for Perforin Antibody, anti-human, REAfinity™

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with REA1061
dG9	++
gG9	++
B-D48	-
Cells were incubated with an excess of purified unconjugated Perforin (REA1061) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Perforin Antibody, anti-human, REAfinity™

Overview

Clone REA1061 recognizes the human perforin, a 70 kDa cytolytic protein, which is expressed by cytotoxic T lymphocytes and natural killer (NK) cells, and which is stored in cytoplasmic granules. Upon cell activation perforin is released and functions as one of the major effector molecule to mediate killing of target cells.

Additional information: Clone REA1061 displays negligible binding to Fc receptors.

Alternative names

PRF1, FLH2, HPLH2, P1, PFN1, PFP

Detailed product information

Technical specifications

Clone	REA1061
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (I), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	Perforin
Alternative names of antigen	PRF1, FLH2, HPLH2, P1, PFN1, PFP
Molecular mass of antigen [kDa]	59
Distribution of antigen	NK cells, T cells
Entrez Gene ID	5551
RRID	AB_2733687, AB_2733890, AB_2733891, AB_2733444, AB_2733445, AB_2733924, AB_2733925, AB_2889728, AB_2922362, AB_2922353, AB_2922361, AB_2922352, AB_2922363, AB_2922354, AB_2733686

Resources for Perforin Antibody, anti-human, REAfinity™

Certificates

Please follow this

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References for Perforin Antibody, anti-human, REAfinity™

Publications

Voskoboinik, I. et al. (2015) Perforin and granzymes: function, dysfunction and human pathology. Nat. Rev. Immunol. 15(6): 388-400

Applications

Extended validation