PepTivator® WT1, human

PepTivator
®
WT1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the WT1 protein (UniProt ID: P19544).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific T cells, as peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
Quantitative, phenotypical, or functional analysis of

Specifications for
PepTivator
®
WT1
, human

Overview

PepTivator
®
WT1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the WT1 protein (UniProt ID: P19544).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific T cells, as peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
Quantitative, phenotypical, or functional analysis of WT1–specific T cell immunity can provide important information on the natural course of immune responses.

Detailed product information

Background information

Wilms tumor 1 (WT1), a zinc-finger transcription factor, is expressed during normal ontogenesis. Expression is absent or restricted to a very low level in adult healthy tissue. The WT1 protein is abundantly found in various types of hematological maligancies and solid tumors. It is a key molecule for tumor proliferation, inducing spontaneous humoral and cytotoxic T cell responses, and has become an attractive target for anticancer immunotherapy.

Downstream applications

The
in vitro
stimulation of WT1–specific T cells with PepTivator WT1 causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of WT1–specific T cells.
  • Detection and analysis of WT1–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable WT1–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines for research on immunotherapy.
  • Generation of WT1–specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
  • Pulsing of antigen-presenting cells for research on dendritic cell vaccination.

Resources for
PepTivator
®
WT1
, human

Documents and Protocols

Brochures/posters

PepTivator® Peptide Pools - Standardize your T cell assays

Related products for
PepTivator
®
WT1
, human

2 products available
130-092-658

CD154 MicroBead Kit, human

The CD154 MicroBead Kit has been developed for positive selection of activated CD154+ ...

130-093-476

CD137 MicroBead Kit, human

The CD137 MicroBead Kit was developed for the positive selection of human CD137+ cells from PBMCs. ...

Seems like you are coming from USA!
Do you want to visit our website in your country?

Copyright © 2023 Miltenyi Biotec and/or its affiliates. All rights reserved.