PepTivator® Insulin, human

PepTivator
®
Insulin is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of human insulin (UniProt ID: P01308).
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Specifications for
PepTivator
®
Insulin
, human

Overview

PepTivator
®
Insulin is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of human insulin (UniProt ID: P01308).
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Detailed product information

Background information

Insulin is expressed by pancreatic β cells and is a major target of autoreactive T cell responses in type 1 diabetes.

Downstream applications

PepTivator Insulin – research grade has been specifically developed for efficient
in vitro
stimulation of insulin–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications, including:
  • Detection and analysis of insulin–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable insulin–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of insulin–specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.

Resources for
PepTivator
®
Insulin
, human

Documents and Protocols

Brochures/posters

PepTivator® Peptide Pools - Standardize your T cell assays

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®
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