PepTivator® gp100/Pmel 17, human

The PepTivator
®
gp100/Pmel 17 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acids (aa) overlap, covering the complete sequence of this antigen (UniProt ID: P40967).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells, as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative, phenotypical, or

Specifications for
PepTivator
®
gp100/Pmel 17
, human

Overview

The PepTivator
®
gp100/Pmel 17 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acids (aa) overlap, covering the complete sequence of this antigen (UniProt ID: P40967).
PepTivator peptide pools have been developed for the efficient
in vitro
stimulation of antigen–specific CD4
+
and CD8
+
T cells, as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications.
1
Quantitative, phenotypical, or functional analysis of gp100/Pmel 17–specific T cell immunity can provide important information on the natural course of immune responses.

Detailed product information

Background information

Gp100/Pmel 17 belongs to the melanoma-associated antigens of the group of differentiation antigens. Other members of this group are, for example, tyrosinase and Melan-A/MART-1. Gp100/Pmel 17 is expressed on normal melanocytes, but is also present in melanoma. CD4
+
and CD8
+
T lymphocytes recognizing gp100/Pmel 17 have been identified in melanoma patients.
2

Downstream applications

The
in vitro
stimulation of gp100/Pmel 17 CD4
+
and CD8
+
T cells with PepTivator gp100/Pmel 17 causes secretion of effector cytokines and upregulation of activation markers, which then allow the detection and isolation of gp100/Pmel 17–specific T cells.
  • Detection and analysis of gp100/Pmel 17–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
  • Isolation of viable gp100/Pmel 17–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of gp100/Pmel 17–specific CD4+ and CD8+ effector/memory T cells for research on immunotherapy and vaccination
  • Pulsing of antigen-presenting cells, for example, for research in dendritic cell vacchination..

References for
PepTivator
®
gp100/Pmel 17
, human

Publications

  1. Kiecker, F. et al. (2004) Analysis of antigen-specific T-cell responses with synthetic peptides--what kind of peptide for which purpose? Hum. Immunol. 65: 523-536
  2. Boon, T. et al. (2006) Human T cell responses against melanoma. Annu. Rev. Immunol. 24: 175-208

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PepTivator
®
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, human

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