Flow cytometric comparison of different clones for Nur77. Thymocytes from C57BL/6 were stimulated with 20ng/ml Phorbol-12-myristat-13-acetat (PMA) and 500ng/ml Ionomycin for 2 hours. Stimulated cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Nur77 antibodies. As a control, Nur77 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Nur77. Thymocytes from C57BL/6 were stimulated with 20ng/ml Phorbol-12-myristat-13-acetat (PMA) and 500ng/ml Ionomycin for 2 hours. Stimulated cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Nur77 antibodies. As a control, Nur77 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Nur77. Thymocytes from C57BL/6 were stimulated with 20ng/ml Phorbol-12-myristat-13-acetat (PMA) and 500ng/ml Ionomycin for 2 hours. Stimulated cells were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Transcription Factor Staining Buffer Set followed by intracellular staining with Nur77 antibodies. As a control, Nur77 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.