Naive CD8a
⁺
T Cell Isolation Kit
, mouse

Name: Naive CD8a+ T Cell Isolation Kit, mouse
Availability: InStock

Naive CD8a
⁺
T Cell Isolation Kit
, mouse

The Naive CD8a

⁺

T Cell Isolation Kit was developed for the isolation of untouched naive CD8a

⁺

T cells from mouse spleen and lymph node cells.

Product data and images for Naive CD8a
⁺
T Cell Isolation Kit
, mouse

Figures

Figure 1

Naive CD8a

⁺

T cells were isolated from a single-cell suspension from mouse spleen using the Naive CD8a

⁺

T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD3ε-APC (# 130-092-977), CD8a-VioBlue (# 130-094-360), CD44-FITC (# 130-096-842), and CD62L-PE (# 130-091-756) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction

Total lymphocytes	Gated on CD3 ⁺ CD8a ⁺ T cells
View details Naive CD8a ⁺ T Cell Isolation Kit , mouse Figure 1 Naive CD8a ⁺ T cells were isolated from a single-cell suspension from mouse spleen using the Naive CD8a ⁺ T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD3ε-APC (# 130-092-977), CD8a-VioBlue (# 130-094-360), CD44-FITC (# 130-096-842), and CD62L-PE (# 130-091-756) and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details Naive CD8a ⁺ T Cell Isolation Kit , mouse Figure 1 Naive CD8a ⁺ T cells were isolated from a single-cell suspension from mouse spleen using the Naive CD8a ⁺ T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD3ε-APC (# 130-092-977), CD8a-VioBlue (# 130-094-360), CD44-FITC (# 130-096-842), and CD62L-PE (# 130-091-756) and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Isolated naive CD8a⁺ T cells

Total lymphocytes

Gated on CD3

⁺

CD8a

⁺

T cells

Figure 1

Naive CD8a

⁺

T cells were isolated from a single-cell suspension from mouse spleen using the Naive CD8a

⁺

T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD3ε-APC (# 130-092-977), CD8a-VioBlue (# 130-094-360), CD44-FITC (# 130-096-842), and CD62L-PE (# 130-091-756) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

Naive CD8a

⁺

T cells were isolated from a single-cell suspension from mouse spleen using the Naive CD8a

⁺

T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD3ε-APC (# 130-092-977), CD8a-VioBlue (# 130-094-360), CD44-FITC (# 130-096-842), and CD62L-PE (# 130-091-756) and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Naive CD8a
⁺
T Cell Isolation Kit
, mouse

Overview

The Naive CD8a

⁺

T Cell Isolation Kit was developed for the isolation of untouched naive CD8a

⁺

T cells from mouse spleen and lymph node cells.

Detailed product information

Detailed separation procedure

For magnetic depletion of memory CD8

⁺

T cells and non-CD8

⁺

T cells, single cell suspension is incubated with a cocktail of biotinylated CD45R, CD4, CD11b, CD11c, CD19, CD44, CD49b, CD105, Ter-119, MHC II, and anti-TCRγ/δ antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.

Downstream applications

Naive CD8a

⁺

T cells can be used for further

in vitro

analysis of T cell differentiation or adoptive transfer experiments.

Columns

LS, XS, or autoMACS

^®

Columns.

References for Naive CD8a
⁺
T Cell Isolation Kit
, mouse

Publications

Hinrichs, C. S. et al. (2009) Adoptively transferred effector cells derived from naive rather than central memory CD8+ T cells mediate superior antitumor immunity. Proc. Natl. Acad. Sci. U.S.A. 106(41): 17469-17474
Kamimura, D. and Bevan, M. J. (2007)
Naive CD8
⁺
T cells differentiate into protective memory-like cells after IL-2 anti IL-2 complex treatment
in vivo
.
J. Exp. Med. 204(8): 1803-1812

Data and images