The IL-17 Secretion Assay was developed for the sensitive detection of mouse IL-17A–secreting cells.

Data and images for Mouse IL-17 Secretion Assay – Detection Kits

Figures

Figure 1

Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were stained by using the Mouse IL-17 Secretion Assay – Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.
Stimulated sample
Unstimulated control
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Figure 1

Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were stained by using the Mouse IL-17 Secretion Assay – Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.
View details

Figure 1

Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were stained by using the Mouse IL-17 Secretion Assay – Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.

Specifications for Mouse IL-17 Secretion Assay – Detection Kits

Overview

The IL-17 Secretion Assay was developed for the sensitive detection of mouse IL-17A–secreting cells.

Detailed product information

Background information

Interleukin 17 (IL-17) is a family of cytokines that play a central role in adaptive immunity as well as autoinflammatory disorders.
1
Data from several mouse models suggest that IL-17 plays a key role in the host defense against certain extracellular bacterial infections.
Recently, a new mouse T helper cell subset has been identified, which is dinstinct from Th1 and Th2 cells: IL-17–producing CD4
+
T helper (Th17) cells. Th17 cells preferentially produce IL-17A, IL-17F, IL-21, and IL-22. Receptors for IL-17 and IL-22 are expressed on various epithelial tissues, thus Th17 cells are crucial for the cross-talk between immune system and tissues.
2
It is now established that Th17 cells are responsible for driving autoimmune inflammation.
After stimulation of mouse cells, IL-17 is not only secreted by CD4
+
T cells, but also by CD8
+
T cells, γδ T cells, NK cells, and granulocytes.

Resources for Mouse IL-17 Secretion Assay – Detection Kits

References for Mouse IL-17 Secretion Assay – Detection Kits

Publications

  1. Boussiotis, V. A. et al. (2012) Runx1 and Runx3 are involved in the generation and function of highly suppressive IL-17-producing T regulatory cells. PLoS One 7(9)
  2. Ishikawa, H. et al. (2017) JunB is essential for IL-23-dependent pathogenicity of Th17 cells. Nat Commun. 8
  3. Weaver, C. T. et al. (2007) IL-17 family cytokines and the expanding diversity of effector T cell lineages. Annu. Rev. Immunol. 25: 821-852
  4. Ouyang, W. et al. (2008) The biological functions of T helper 17 cell effector cytokines in inflammation. Immunity 28: 454-467

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