MHC Class II Antibody, anti-mouse
Clone:
M5/114.15.2
Type of antibody:
Primary antibodies
Isotype:
rat IgG2bκ
Applications:
FC, MICS, IF, IHC
Alternative names:
H2-AB1, Abeta, H-2Ab, H2-Ab, I-Abeta, IAb, Ia-2, Ia2, Rmcs1

Extended validation for MHC Class II Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with M5/114.15.2
REA813++
2G9++
REAL362n/a
Cells were incubated with an excess of purified unconjugated MHC Class II (M5/114.15.2) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for MHC Class II. Splenocytes from C57BL/6 were stained with MHC Class II antibodies and with a suitable counterstaining. As a control, MHC Class II antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MHC Class II. Splenocytes from C57BL/6 were stained with MHC Class II antibodies and with a suitable counterstaining. As a control, MHC Class II antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MHC Class II. Splenocytes from C57BL/6 were stained with MHC Class II antibodies and with a suitable counterstaining. As a control, MHC Class II antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MHC Class II. Splenocytes from C57BL/6 were stained with MHC Class II antibodies and with a suitable counterstaining. As a control, MHC Class II antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MHC Class II. Splenocytes from C57BL/6 were stained with MHC Class II antibodies and with a suitable counterstaining. As a control, MHC Class II antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MHC Class II. Splenocytes from C57BL/6 were stained with MHC Class II antibodies and with a suitable counterstaining. As a control, MHC Class II antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MHC Class II. Splenocytes from C57BL/6 were stained with MHC Class II antibodies and with a suitable counterstaining. As a control, MHC Class II antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for MHC Class II. Splenocytes from C57BL/6 were stained with MHC Class II antibodies and with a suitable counterstaining. As a control, MHC Class II antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for MHC Class II Antibody, anti-mouse

Overview

The Anti-MHC Class II antibody reacts with the MHC class II alloantigens I-Ab, I-Aq, I-Ad, I-Ed, and I-Ek that are expressed by most common inbred mouse strains, for example, C57BL/6, BALB/c, or 129/SvEv 1. MHC class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes/macrophages, B cells in lymphoid and non-lymphoid tissue, thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow.

Alternative names

H2-AB1, Abeta, H-2Ab, H2-Ab, I-Abeta, IAb, Ia-2, Ia2, Rmcs1

Detailed product information

Technical specifications

CloneM5/114.15.2
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies
Speciesmouse
AntigenMHC Class II
Alternative names of antigenH2-AB1, Abeta, H-2Ab, H2-Ab, I-Abeta, IAb, Ia-2, Ia2, Rmcs1
Molecular mass of antigen [kDa]27
Distribution of antigendendritic cells, monocytes, macrophages, epithelial cells, stem cells, cancer stem cells, hematopoietic stem and progenitor cells
Entrez Gene ID14961
RRIDAB_2802021, AB_2802055, AB_2811551, AB_2660055, AB_2660056, AB_2660063, AB_2660064, AB_2660065, AB_2660066, AB_2734108

Resources for MHC Class II Antibody, anti-mouse

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for MHC Class II Antibody, anti-mouse

Publications

  1. Bhattacharya, A. et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol 127(6): 2488-2495

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