Mesothelin Antibody, anti-human, REAfinity™

Name: Mesothelin Antibody, anti-human, REAfinity™
Availability: InStock

Mesothelin Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA1057

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

FC

Alternative names:

CAK1, MPF, MSLN

Flow cytometry applications forMesothelin Antibody, anti-human, REAfinity™

APC
Biotin
FITC
PE

Conjugate:

APC

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-118-169, 130-118-096

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Capan-2 cells were stained with Anti-Mesothelin antibodies or with the corresponding REA Control (S) antibodies (left peak) and analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Extended validation for Mesothelin Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Mesothelin. Capan-2 cells were stained with Mesothelin antibodies and plotted against the side scatter. As a control, Mesothelin antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using Mesothelin (REA1057). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using Mesothelin (REA1057). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for Mesothelin Antibody, anti-human, REAfinity™

Overview

Clone REA1057 recognizes the human mesothelin antigen, a membrane glycoprotein also known as CAK1 or pre-pro-megakaryocyte-potentiating factor. Mesothelin is expressed on mesothelial cells and overexpressed on different tumors, e.g., mesotheliomas and adenocarcinomas. Mesothelin is a precursor protein which has functions in cellular adhesion.

Additional information: Clone REA1057 displays negligible binding to Fc receptors.

Alternative names

CAK1, MPF, MSLN

Detailed product information

Technical specifications

Clone	REA1057
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (S), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	Mesothelin
Alternative names of antigen	CAK1, MPF, MSLN
Molecular mass of antigen [kDa]	35
Distribution of antigen	tumor cells, other
Entrez Gene ID	10232
RRID	AB_2733681, AB_2733876, AB_2733877, AB_2733435, AB_2733436, AB_2733558, AB_2733559, AB_2733680

Resources for Mesothelin Antibody, anti-human, REAfinity™

Certificates

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References for Mesothelin Antibody, anti-human, REAfinity™

Publications

Iwahori, K. et al. (2008) Megakaryocyte potentiating factor as a tumor marker of malignant pleural mesothelioma: evaluation in comparison with mesothelin. Lung Cancer 62(1): 45-54

Applications

Extended validation