Name: MACSprep™ Chimerism CD34 MicroBead Kit, human
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Product data and images for MACSprep™ Chimerism CD34 MicroBead Kit, human

Figures

Figure 1

**Protocol overview:**
Isolation of CD34
⁺
cells from whole blood.

Figure 1

Protocol overview:

Isolation of CD34

⁺

cells from whole blood.

Figure 2

Isolation of CD34

⁺

cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue

^®

and analyzed by flow cytometry using the MACSQuant

^®

Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34

⁺

cells after separation is defined by percentage of CD34

⁺

cells (viable CD45d

^im/+

CD34

⁺

cells) amongst leukocytes (viable CD45

^dim/+

cells). The CD34

⁺

cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34

⁺

cells varies due to the variation of human samples and experimental set-up.

Before separation
View details MACSprep™ Chimerism CD34 MicroBead Kit, human Figure 2 Isolation of CD34 ⁺ cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 ⁺ cells after separation is defined by percentage of CD34 ⁺ cells (viable CD45d ^im/+ CD34 ⁺ cells) amongst leukocytes (viable CD45 ^dim/+ cells). The CD34 ⁺ cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 ⁺ cells varies due to the variation of human samples and experimental set-up.
After separation with one column	After separation with two columns
View details MACSprep™ Chimerism CD34 MicroBead Kit, human Figure 2 Isolation of CD34 ⁺ cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 ⁺ cells after separation is defined by percentage of CD34 ⁺ cells (viable CD45d ^im/+ CD34 ⁺ cells) amongst leukocytes (viable CD45 ^dim/+ cells). The CD34 ⁺ cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 ⁺ cells varies due to the variation of human samples and experimental set-up.	View details MACSprep™ Chimerism CD34 MicroBead Kit, human Figure 2 Isolation of CD34 ⁺ cells from a whole blood sample from a healthy donor using the MACSprep™ Chimerism CD34 MicroBead Kit, two LS Columns, a MACSmix™ Tube Rotator and a MidiMACS™ Separator. Cells were fluorescently stained with CD34-FITC and CD45-VioBlue ^® and analyzed by flow cytometry using the MACSQuant ^® Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. The purity of CD34 ⁺ cells after separation is defined by percentage of CD34 ⁺ cells (viable CD45d ^im/+ CD34 ⁺ cells) amongst leukocytes (viable CD45 ^dim/+ cells). The CD34 ⁺ cells content of the isolated fraction with one column is typically around 69.1 ± 5.8% (mean ± SD); 90.8 ± 3.4% (mean ± SD) with two columns.The purity of CD34 ⁺ cells varies due to the variation of human samples and experimental set-up.

Specifications for MACSprep™ Chimerism CD34 MicroBead Kit, human

Overview

MACSprep™ Chimerism CD34 MicroBead Kit has been developed for the fast isolation of CD34

⁺

cells from freshly drawn anticoagulated whole blood without density gradient centrifugation nor red blood cell lysis.

Detailed product information

Background information

The isolation of CD34

⁺

cells is performed with one labeling step without washing afterwards and in a two-step separation procedure. During the first isolation step red blood cells are aggregated and sedimented. In a second step, CD34

⁺

cells are magnetically labeled with MACSprep Chimerism CD34 MicroBeads. Then, the cell suspension is loaded onto a MACS

^®

Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled CD34

⁺

cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of CD34

⁺

cells. After removing the column from the magnetic field, the magnetically retained CD34

⁺

cells can be eluted as the positively selected cell fraction.

Resources for MACSprep™ Chimerism CD34 MicroBead Kit, human

Documents and Protocols

Brochures/posters

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